聚β-羟基丁酸酯对卤虫幼体的强化作用

Artemia nauplii enrichment with polyβ-hydroxybutyrate

摘要:

通过投喂实验，探讨了不同浓度聚β羟基丁酸酯PHB（0，50，100，150，200，400和800 mg/L）和不同强化培养时间（24 h，48 h和72 h）对卤虫生长和存活的影响。实验采用超声波处理方法将不溶于水的PHB粉末分散成颗粒较小的均匀悬浮液（直径为10～50 μm），并按上述浓度投喂初孵卤虫。卤虫无节幼体初始密度为100个/200 mL。结果表明，当PHB浓度为100 mg/L时，卤虫成活率显著高于其它组，且成活率随PHB浓度的增加而明显降低，24 h卤虫成活率明显高于48 h和72 h（P<0.05），同时72 h卤虫的体长也显著高于其它实验组（P<0.05）。定量测定结果表明，24 h内卤虫体内PHB含量随强化时间的延长而增加，强化24 h卤虫体内PHB 含量最高，达到卤虫干重的10.73%。综上所述，我们提出利用卤虫生物包裹的方法将PHB应用于水产育苗，以100 mg/L PHB浓度强化卤虫幼体24 h效果最佳。

Abstract:

Effect of polyβ-hydroxybutyrate (PHB) on survival and growth of Artemia was investigated. Various levels of PHB concentration (0, 50, 100, 150, 200, 400 and 800 mg/L, respectively) and different enrichment periods (24 h, 48 h and 72 h, respectively) were applied during Artemia enrichment in two separate trials. PHB suspension was prepared with ultrasonicator to make the particle size smaller (ranging from 10 to 50 μm) and fed to the newlyhatched Artemia nauplii according to the designed concentration. The initial density of Artemia nauplii was 100 ind/200 mL. The results showed that survival of Artemia enriched with 100 mg/L PHB for 24 h was significantly higher than that of other treatments, which was reduced remarkably when Artemia were enriched with other PHB concentrations and longer periods (48 h and 72 h) (P<0.05). And the 72 hgrowth of Artemia enriched with 100 mg/L PHB was also significantly higher than that of other groups (P<0.05). The result of quantitative determination indicated that, within 24 h enrichment, PHB content in Artemia increased along with enrichment time and reached the highest at 24 h, accounting for 10.73% of Artemia dry weight. In conclusion, we proposed that PHB can be applied efficiently in larviculture through Artemia enrichment at a level of 100 mg/L PHB for 24 h.