宠物龟眼炎的病原鉴定及临床药物治疗的研究

Identification of pathogen from pet chelonian with ophthalmia and the clinic therapy

摘要:

眼炎是宠物龟疾病中一种常见的疾病，具有闭眼、眼睑红肿、瞬膜异位等症状。从患有眼炎的宠物龟眼部分离出菌株A5，经人工回归感染试验确定其为眼炎的病原菌。综合该菌在形态、生理生化特征及16S rDNA测序分析等检验结果，确定A5菌株为荧光假单胞菌（Pseudomonas fluorescens bv.C）。药敏试验证实，该菌对氧氟沙星、庆大霉素、妥布霉素、氧哌嗪青霉素等抗生素敏感。对40个眼炎病例进行分组药物治疗，比较治疗方案，结果显示：氧氟沙星药效优于氯霉素与磺胺醋酰钠，养殖水温25 ℃治疗效果优于5 ℃，眼科手术剔除分泌物可缩短治疗时间。增加给药次数至6次/d、增加肌肉注射，疗效无显著性差异。此研究结果将为宠物龟眼科疾病的诊断与治疗起到指导性作用。

Abstract:

Symptoms of ophthalmia of pet chelonian included eye closed, eyelid got red and membranae nictitans moved. It is a common disease on pet chelonian. A dominant bacterial strain isolated from the sick chelonian’s eye is assigned as the strain A5. The artificial infection test proved that the isolated A5 was the pathogenic bacterium that caused the disease. Synthesizing the morphology, physiological and biochemical characterization, and 16S rDNA of A5 was sequenced, the results showed that strain A5 exhibited the highest levels of similarity to the Pseudomonas fluorescens bv.C. The drug sensitivity test of strain A5 revealed that the pathogen was sensitive to drugs such as ofloxacin, gentamicin, tenebrimycin and piperacillin. The forty ophthalmic pet chelonians were divided into 8 groups to be treated and the efficacy analyzed in different treatments. The results showed that the drug effect of ofloxacin was better than sulfacetamide sodium and chloramphenicol. The curative effect of sick chelonian treated at 25 ℃ was better than at 5 ℃, and doing surgery was good for the cure of ophthalmia. Adding the dose of drug and intramuscular injection had no significant difference. The study could be helpful for clinic diagnosis in pet chelonian disease.