甜菜碱对奥尼罗非鱼生长、体脂含量及血清生化指标的影响

Effects of dietary Betaine on growth， fat content and biochemical indexs in blood of tilapia （Oreochromis niloticus×Oreochrmis aureus）

摘要:

取奥尼罗非鱼（Oreochromis niloticus×Oreochrmis aureus）750尾，随机分为5组，分别饲喂甜菜碱（Betaine）添加量为0、0.15%、0.30%、0.45%和0.60%的饲料，饲养65 d，以研究甜菜碱对奥尼罗非鱼生长、体脂含量及血清生化指标的影响。结果表明：当甜菜碱添加量为0.30%~0.60%时，罗非鱼的增重率较对照组分别提高19.52%、16.63%和21.38%；饲料系数分别降低25.55%、20.44%和29.93%；肝体比降低9.29%、38.57%和29.29%；血清中甘油三酯含量较对照组分别降低66.28%、55.81%和80.23%；淀粉酶活性降低88.78%、37.59%和62.41%；白蛋白和总蛋白含量升高，胆固醇含量显著降低；谷丙转氨酶、谷草转氨酶、乳酸脱氢酶活性均有所降低。在本研究条件下，建议奥尼罗非鱼饲料中甜菜碱的适宜添加量为0.45%~0.60%。

Abstract:

750 juvenile tilapia（Oreochromis niloticus×Oreochrmis aureu.s） were randomly allotted to five groups, and were fed diets supplemented with different level Betaine (0、0.15%、0.30%、0.45% and 0.60%) for 65 d to investigate the effects of Betaine on growth, fat content and biochemical indexs of blood of tilapia. The results showed that tilapia’ WGR were 19.52%、16.63% and 21.38% higher than that of control group (P<0.05) respectively when tilapia feed were added with 0.30%-0.60% Betaine; FRC reduced by 25.55%、20.44 % and 29.93%（P<0.05）respectively; the HSI reduced by 9.29%、38.57% and 29.29% (P<0.05); the contents of TG in Tilapia’s plasma reduced by 66.28%、55.81% and 80.23% (P<0.05) respectively; the activities of AMY in Tilapia’s plasma reduced by 88.78%、37.59% and 62.41% (P<0.05); the contents of ALB and TP in Tilapia’s plasma were improved, the contents of CHOL in Tilapia’s plasma reduced; the activities of LDH、ALT and AST in Tilapia’s plasma reduced respectively. Therefore, under this research condition, it is suggested that the supplement of Betaine in diets of juvenile tilapia was the best at 0.45%~0.60%.