乌苏里江流域尖吻细鳞鲑及钝吻细鳞鲑群体遗传多样性分析

Assessing genetic diversity of the Brachymystax lenok and Brachymystax tumernsis populations in Ussuri River

摘要:

采用扩增片段长度多态性(Amplified Fragment Length Polymorphism,AFLP)技术对乌苏里江尖吻和钝吻两种形态的细鳞鲑各10个个体进行了遗传多样性分析。结果显示,12对选择性扩增引物共扩增得到433个位点,其中多态性位点322个,多态性百分比为74.36%。且在12对选择性扩增引物中,引物B3的聚丙烯电泳图谱显示,在130 bp和136 bp处,钝吻群体有两条特异性扩增条带产生。对2个群体的Shannon多样性指数,Nei氏基因多样性等各项参数进行了相关分析。尖吻和钝吻细鳞鲑个体UPGMA聚类树分为两个明显的大分支,群体的遗传相似度为0.836 7,遗传距离为0.178 3,而钝吻细鳞鲑的各项多样性指数均略高于尖吻细鳞鲑。

Abstract:

Lenok(Brachymystax lenok),which was a kind of Chinese famous endemic fish,had undergone a dramatic decline for the impacts of human and environment.In this paper,the genetic diversity of the twenty individuals of B.lenok and B.tumernsis populations,which from the Ussuri River,were analyzed using the AFLP method.In total,322 polymorphic loci of 433 were amplified with 12 primer pairs and the percentage of polymorphic loci was 74.36%.In the 130 and 136 bp,there were two differential bands of the B.tumernsis' ...