八个品种金鱼及野生鲫线粒体控制区遗传差异和亲缘关系的研究

The genetic diversity and phylogenetic relationships of mitochondrial D-Loop partial sequences in eight representative varieties of goldfishes(Carssius auratus var) and wild crucian carp(Carssius auratus)

摘要:

采用PCR技术扩增了红草金鱼(RC),红文鱼(RF),红顶白高头(WR),白珍珠(WP),红狮头(RT),红龙睛蝶尾(MB),墨龙睛(BM),红水泡(RB)八个品种金鱼和野生鲫(WC)线粒体DNA的D-Loop区部分序列,PCR产物经纯化、测序、同源序列比对后获得长度为699 bp的核苷酸序列,在129个样本中共检测到了17个单倍型,9个品种共享1种单倍型,5个品种的金鱼(BM,MB,WR,RF,RC)检测到各自独有的单倍型。遗传距离和聚类分析结果显示6个品种金鱼(WP,BM,MB,RT,WR,RB)首先聚在一起,然后依次与红文鱼(RF)、红草金鱼(RC)、野生鲫(WC)聚在一起。金鱼首先演化为草系金鱼,草系金鱼在演变为文系中较为古老的品种之后,经过进一步的变异,分化出金鱼其他各个品种。

Abstract:

Genetic diversity and phylogenetic relationship in goldfish(Carssius auratus var) were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp.The eight representative varieties were Red Common Goldfish(RC),Red Fantail(RF),White Oranda with Red Carp(WR),White Pearlscale(WP),Red Tigerhead(RT),Black Moor(BM),Red Moor with Butterfly Tail(MB) and Red Bubble-eye with Dorsal Fin(RB).699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism.Sequences data showed that all 129 sequences were grouped into 17 haplotypes.Nine representative varieties shared same haplotype.Five representative varieties of goldfishes(BM,MB,WR,RF,RC)had haplotypes respectively.The results from the analyses of genetic distance and cluster analysis showed that six representative varieties(WP,BM,MB,RT,WR,RB) were clustered together first,which then clustered together with RF and finally clustered together with RC and WC.Wild common carp evolved to common goldfish first,and then common goldfish evolved to some primitive varieties of fantail goldfish,then differentiated to other representative varieties.