池蝶蚌与三角帆蚌及其杂种F1的同工酶鉴别

Isozyme identification of Hyriopsis schlegeli,Hyriopsis cumingii and their reciprocal hybrids F1

摘要:

通过对外来种池蝶蚌(C)、三角帆蚌(S)及其正反杂种F1的肝脏同工酶进行垂直板聚丙烯酰胺凝胶电泳,分析了肝脏的SOD和EST同工酶谱带。结果表明,这两种蚌及其正反杂种F1同工酶的表达既呈现出一些相似的特征,又有明显差异。这四种蚌中分别有2~5个SOD位点,其中SOD-1为一强带且仅见于F1SC中,SOD-2在F1SC中为强带,但在F1CS中较弱,而其双亲中则无该带;SOD-3为一强带均保守的见于四种蚌中,SOD-4为一强带但仅在杂种F1SC中无表达,SOD-5为一强带但仅在池蝶蚌中未检到;在两亲本与其杂种F1中分别检测到4~6个EST同工酶位点,其中EST-4仅见于杂种F1SC中,EST-5在杂种F1CS中无表达,其余均有不同强弱的表达。

Abstract:

In the paper,isozymes(SOD,EST)from liver of alien species Hyriopsis schlegeli(C),H.cumingii(S) and their reciprocal hybrids F_1 were analyzed by vertical polyacrylamide gel electrophoresis.The results indicated that phenotype polymorphism in the isozymes electrophoresis meanwhile some common characters were observed among H.schlegeli,H.cumingii and their reciprocal hybrids F_1.There are 2-5 SOD loci in parental mussels and their reciprocal hybrids F_1.A strong activity of SOD-1 is visible only in F_1SC,and about SOD-2,a strong activity band in F_1SC,while a weak band in F_1CS but no band in parent is observed,respectively.There is a strong conservative band SOD-3 in all mussels and a strong band SOD-4 except in F_1SC,and a strong band SOD-5 except in Hyriopsis schlegeli are visible.4-6 EST loci are visible in the 2 species mussels and their reciprocal hybrids F_1 and all loci have a varying degree of activity but EST-4 invisible in F_1SC and EST-5 invisible in F_1CS.