Abstract:The structure and development of male reproductive system in the freshwater prawn, Macrobrchium nipponense, was studied with paraffin-section method from September 1992 to Auguest 1993, The male reproductive system consists of paired testes, vas deferens, terminae ampulla and male gonopore. The testes contain great number of seminiferous tubules. Each tubule is made up of germinative epithelium and tubule liner.The germinative epithelium produces various spermatogenis cell areas. The vas deferens can be divided morphologically into three regions: the anterior, the middle belonged to high convolution, and the posterior. The vas deferens is composed of columnar epithelium surrounded by striated muscle. Two types of cells,high columnar cells and low columnar cells can be distinguished in the epithelium of the vas deferens which can secrete spermatophore matrixes. The terminal ampulla is highly muscularized. The development of the testes has seasonal periodicity.