两种农药对泥鳅红细胞微核和核异常的诱导

INDUCTION OF MICRONUCLEI AND NUCLEAR ANOMALIES IN ERYTHROCYTES OF LOACH BY TWO PESTICIDES

摘要:

本研究以泥鳅作为试验鱼，在测定甲胺磷对其96小时半致死浓度的基础上，在半致死浓度和安全浓度之间设置五个浓度组，研究了甲胺磷和敌枯双各个浓度组对泥鳅红细胞微核形成和核异常的影响。试验结果表明，两种农药都能诱导泥鳅红细胞微核细胞率和核异常率的提高。在一定浓度范围内，微核细胞率与农药浓度呈正相关；但当浓度过高时，微核细胞率反而随浓度的升高而降低。而总核异常率则总是随农药浓度的升高而提高。此外，研究还发现，随着农药浓度的升高，微核细胞率出现高峰的时间也提前。

Abstract:

Pesticides causing damages to the erythrocyte nucleus of loach, Misgurnus anguillicaudatus Cantor, were reported in this paper. On the basis of determining 96hr LC50of methamidophos, the effects of two pesticides on micronuclei and nuclear anomalies in erythrocytes of loach at five different concentration between LC50 and the safety were discussed. The experimental results indicated that the two pesticides could increase the frequency of micronucleated erythrocytes and the rate of nuclear anomalies. At the certain range of the pesticide concentration, the frequency of micronucleated erythrocytes showed a dosedependent elevation. However, it decreased with the increase of pestcide concentration when the concentration was over a suitable range. But the rate of total nuclear anomalies showed a significant dose-dependent increases in the test. In addition, with the increase of the doses for each pesticide, the hight frequency of micronucleated erythrocytes appeared also earlier.